Death Receptor 5 Antisense Long Noncoding RNA and Cisplatin Modulate Macromolecular and Metabolic Changes in HeLa Cells. Death Receptor 5 Antisense Long Noncoding RNA and Cisplatin Modulate Macromolecular and Metabolic Changes in HeLa Cells

We have performed a transcriptomics study in which we first apply cisplatin to HeLa cells. Total RNA was isolated from control as well as treated cells and apoptosis was confirmed by Annexin V and 7AAD staining in flow cytometry. Total RNAs were subjected to deep-sequencing to identify differentially expressed mRNAs. We then took advantage of bioinformatic tools to identify which pathways are affected by Cisplatin treatment Overall design: Total RNA was isolated from negative control and Cisplatin (80 uM) treated HeLa cells in triplicate to perform deep-sequencing. Negative controls from GSE160227 data was used as negative control in this study

我们开展了一项转录组学研究，首先将顺铂（cisplatin）处理海拉（HeLa）细胞。从对照组与处理组细胞中分离总RNA，并通过流式细胞术的Annexin V与7AAD染色验证细胞凋亡。随后对总RNA进行深度测序，以鉴定差异表达的mRNA。之后借助生物信息学工具，明确顺铂处理所影响的信号通路。
整体实验设计：本研究设置阴性对照组与80 μM顺铂处理组的海拉细胞，每组设置三次生物学重复，提取总RNA后开展深度测序。本研究采用GSE160227数据集的阴性对照样本作为本次实验的阴性对照。