Genome-wide mapping of H3K27ac marked regions during flower development

Flower developmental stage specific genome-wide H3K27ac loci were mapped in this study. By quantifying the enrichment of H3K27ac modification at each stage, we revealed the dynamic landscape of H3K27ac during flower development. The dynamic of H3K27ac was found to contribute to stage specific gene expression. We found that H3K27ac always locate in proximal region of transcription starting sites but hardly appear in distal intergenic region. In this study, we take advantage of an inducible flower development system,pAP1::AP1-GRap1cal,which allows to manipulate the developmental process of flower formation. Synchronized flower tissue of specific stage could be harvested, which provide the possibility to map stage-specific H3K27ac targets and study the dynamic of this modification across flower development.

本研究针对花发育阶段特异性的全基因组H3K27ac位点开展了定位分析。通过量化各发育阶段H3K27ac修饰的富集程度，本研究揭示了花发育进程中H3K27ac的动态调控图谱。研究表明，H3K27ac的动态变化可调控阶段特异性基因的表达。本研究发现，H3K27ac始终富集于转录起始位点（Transcription Start Site, TSS）的近端区域，极少分布于远端基因间区。本研究借助可诱导花发育系统pAP1::AP1-GRap1cal实现对花器官形成发育过程的精准操控，通过该系统可获得同步化的特定发育阶段花组织样本，为定位阶段特异性H3K27ac靶标以及探究该修饰在花发育全程的动态变化提供了技术支撑。