Molecular Mechanisms of sXbp1 in cultured cardiomyocytes

To understand the molecular mechanisms of spliced Xbp1 (sXbp1), we induced sXbp1 in cultured neonatal rat ventricular myocyte (NRVM). To understand transcriptional change and binding partners of sXbp1, we conducted sXbp1 bulk RNA-Seq and sXbp1 ChIP-Seq.

为阐明剪接型Xbp1（spliced Xbp1, sXbp1）的分子机制，我们在培养的新生大鼠心室肌细胞（neonatal rat ventricular myocyte, NRVM）中诱导了sXbp1的表达。为探究sXbp1的转录变化及其结合伴侣，我们开展了针对sXbp1的批量RNA测序（bulk RNA-Seq）与染色质免疫沉淀测序（ChIP-Seq）实验。