MiR-26 dampens IL-6 production by down-regulating TNF-a/NF-kB signaling through silencing HMGA1 and MALT1 and not by directly targeting IL-6 mRNA

We found NF-kB signaling as a major target of miR-26 based on an analysis of the changes in gene activation by TNF-a in response to alteration of miR-26 levels. Overall design: Transcriptional profiling of genes in response to either a miR-26 mimic or miR-26 antagomir.

本研究通过分析肿瘤坏死因子-α（TNF-α）介导的基因激活变化，并结合miR-26表达水平的改变，发现核因子κB（NF-kB）信号通路是miR-26的主要调控靶点。实验整体设计：对分别经miR-26模拟物（miR-26 mimic）或miR-26拮抗剂（miR-26 antagomir）处理的样本进行基因转录谱分析。