The DNA methylation landscape of human early embryos. Homo sapiens

Our data provided a genome-wide DNA methylation landscape of human early development embryos, including human MII oocytes, sperm, zygotes, 2-cell to 8-cell embryos, morula, blastocyst and postimplantation embryos at single base resolution. Overall design: In total, 44 samples including biological and technical replicates, from 12 different human embryo development stages were analyzed, including two metaphase II oocytes, two zygotes, three first polar bodies, two second polar bodies, four sperm samples, two 2-cell-stage embryos, two 4-cell-stage embryos, three 8-cell-stage embryos, three morulae, three inner cell masses (ICMs) and three trophectoderms (TEs) seperated from late blastocysts, and three post-implantation embryos. In addition, 12 different human embryo development stages were analyzed, including metaphase II oocytes, zygotes, first polar bodies, second polar bodies, sperm samples, 2-cell-stage embryos, 4-cell-stage embryos, 8-cell-stage embryos, morulae, inner cell masses (ICMs) and trophectoderms (TEs) seperated from late blastocysts, and post-implantation embryos.

本数据集提供了人类早期发育胚胎的全基因组DNA甲基化图谱，涵盖人类MII卵母细胞（MII oocytes）、精子、受精卵、2细胞至8细胞期胚胎、桑椹胚、囊胚以及植入后胚胎，分辨率达单碱基水平。
整体实验设计：本研究共分析了来自12种不同人类胚胎发育阶段的44份样本，包含生物学重复与技术重复，具体包括2个中期II卵母细胞、2个受精卵、3个第一极体、2个第二极体、4份精子样本、2个2细胞期胚胎、2个4细胞期胚胎、3个8细胞期胚胎、3个桑椹胚、3个从晚期囊胚分离得到的内细胞团（inner cell masses, ICMs）与3个滋养外胚层（trophectoderms, TEs），以及3个植入后胚胎。
此外，本研究还分析了12种不同人类胚胎发育阶段，涵盖中期II卵母细胞、受精卵、第一极体、第二极体、精子样本、2细胞期胚胎、4细胞期胚胎、8细胞期胚胎、桑椹胚、从晚期囊胚分离得到的内细胞团与滋养外胚层，以及植入后胚胎。