Fertility loss and recovery dynamics after repeated heat stress across life stages in male Drosophila melanogaster: Patterns and processes

Frequent and extreme temperatures associated with climate change pose a major threat to biodiversity, particularly for organisms whose metabolism is strictly linked to ambient temperatures. Many studies have explored thermal effects on survival, but heat-induced fertility loss is emerging as a greater threat to population persistence. However, while evidence is accumulating both juvenile and adult stages heat exposure can impair fertility in their own ways, much less is known about the immediate and longer-term fitness consequences of repeated heat stress across life stages. To address this knowledge gap, we used male Drosophila melanogaster to investigate (i) the cumulative fitness effects of repeated heatwaves across life stages, (ii) the potential of recovery from these heat-exposed, and (iii) the underlying mechanisms. We found individual and combined effects of chronic juvenile and acute adult heat stress on male fitness traits. These effects tended to exacerbate over several days ..., , , \#Fertility Loss and Recovery Dynamics After Repeated Heat Stress Across Life Stages in Male Drosophila melanogaster: Patterns and Processes

\##Authors

Abhishek Meena, Alessio N. De Nardo, Komal Maggu, Sonja Sbilordo, Jeannine Roy, Rhonda R. Snook, Stefan Lüpold

\##Overview

This dataset was collected to analyze fertility, fecundity, hatching success, seminal vesicle area, sperm presence/absence, accessory gland area, and sperm viability (dead and alive sperm) assays in male Drosophila melanogaster. The data is presented in three R Markdown (Rmd) files and three CSV files. The analyses focus on various factors influencing reproductive success under chronic developmental and 4 hours of acute adult temperature conditions.

\##Contents

The dataset includes the following files:

Fitness_Assay.Rmd: This R Markdown file analyzes fertility, fecundity, and hatching success assays using the FitnessAssay.csv file.

FitnessAssay.csv: This CSV file contains the raw data used in the Fitness_Assa...

与气候变化相关的频发极端高温事件正对全球生物多样性构成重大威胁，尤其对那些代谢过程与环境温度严格耦合的生物而言。既往诸多研究已探讨了温度胁迫对生物存活的影响，但热诱导的生育力流失正逐渐被证实是种群存续的更为严峻的威胁。尽管已有越来越多证据表明，幼体与成体阶段的热暴露会通过各自独特的途径损伤生育力，但针对跨生命阶段反复热应激所带来的即时与长期适合度后果，当前学界的认知仍较为匮乏。

为填补这一研究空白，本研究以雄性黑腹果蝇（Drosophila melanogaster）为实验对象，旨在探究三个核心问题：（i）跨生命阶段反复热浪事件对个体适合度的累积效应；（ii）经热暴露后的恢复潜力；（iii）背后潜在的作用机制。

本研究发现，慢性幼体热应激与急性成体热应激对雄性适合度性状存在单独及联合效应，且此类效应往往会随天数推移持续加剧……

# 雄性黑腹果蝇（Drosophila melanogaster）跨生命阶段反复热应激后的生育力流失与恢复动态：模式与过程

## 作者
阿比谢克·米纳（Abhishek Meena）、阿莱西奥·N·德·纳尔多（Alessio N. De Nardo）、科马尔·马古（Komal Maggu）、索尼娅·斯比洛多（Sonja Sbilordo）、让娜·罗伊（Jeannine Roy）、朗达·R·斯努克（Rhonda R. Snook）、斯特凡·吕普尔德（Stefan Lüpold）

## 概述
本数据集用于分析雄性黑腹果蝇的生育力、繁殖力、孵化成功率、储精囊面积、精子存在/缺失状态、副腺面积以及精子活力（死精子与活精子）相关检测结果。数据以3份R Markdown（Rmd）文件与3份CSV文件形式提供。本分析聚焦慢性发育阶段与4小时急性成体温度胁迫条件下，影响繁殖成功率的各类调控因素。

## 数据集内容
本数据集包含以下文件：
Fitness_Assay.Rmd：该R Markdown文件依托FitnessAssay.csv文件，分析生育力、繁殖力与孵化成功率的检测数据。
FitnessAssay.csv：该CSV文件包含用于Fitness_Assa……分析的原始数据。