Testing conditions for ribosome profiling in Drosophila melanogaster. Testing conditions for ribosome profiling in Drosophila melanogaster

Comparison of experimental conditions for ribosome profiling. Overall design: Ribo-seq of Drosophila melanogaster S2 cells from 4 different conditions: untreated or treated with cycloheximide, each undergoing two different RNase I footprinting conditions.

核糖体谱（ribosome profiling）实验条件对比分析。整体实验设计：对黑腹果蝇（Drosophila melanogaster）S2细胞开展核糖体测序（Ribo-seq）实验，共设置4种不同实验条件：未处理组，或经环己酰亚胺（cycloheximide）处理组，每组均采用两种不同的核糖核酸酶I（RNase I）足迹实验条件。