DNA metabarcoding captures subtle differences in forest beetle communities following disturbance
收藏DataCite Commons2021-02-21 更新2025-04-17 收录
下载链接：
https://data.utas.edu.au/metadata/d5969fc2-0eea-4a53-9b2c-f8ff1a7d00ad
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资源简介：
This dataset includes all raw Miseq high-throughput sequencing data, bioinformatic pipeline and R codes that were used in the publication "Liu M, Baker SC, Burridge CP, Jordan GJ, Clarke LJ (2020) DNA metabarcoding captures subtle differences in forest beetle communities following disturbance. Restoration Ecology. 28:1475-1484. DOI:10.1111/rec.13236."
Miseq_16S.zip - Miseq sequencing dataset for gene marker 16S, including 48 fastq files for 24 beetle bulk samples;
Miseq_CO1.zip -Miseq sequencing dataset for gene marker CO1, including 46 fastq files for 23 beetle bulk samples (one sample failed to be sequenced);
nfp4MBC.nf - A nextflow bioinformatic script to process Miseq datasets;
nextflow.config - A configuratioin file needed when using nfp4MBC.nf;
adapters_16S.zip - Adapters used to tag each of 24 beetle bulk samples for 16S, also used to process 16S Miseq dataset when using nfp4MBC.nf;
adapters_CO1.zip - Adapters used to tag each of 24 beetle bulk samples for CO1, also used to process CO1 Miseq dataset when using nfp4MBC.nf;
rMBC.Rmd - R markdown codes for community analyses;
rMBC.zip - Datasets used in rMBC.Rmd.
COI_ZOTUs_176.fasta - DNA sequences of 176 COI ZOTUs.
16S_ZOTUs_156 -DNA sequences of 156 16S ZOTUs.
本数据集包含论文《Liu M, Baker SC, Burridge CP, Jordan GJ, Clarke LJ (2020) DNA宏条形码技术捕捉干扰后森林甲虫群落的细微差异. Restoration Ecology. 28:1475-1484. DOI:10.1111/rec.13236.》中使用的所有Miseq高通量测序原始数据、生物信息学分析流程及R代码。
Miseq_16S.zip——针对16S基因标记的Miseq测序数据集，包含24个甲虫混合样本对应的48个fastq文件；
Miseq_CO1.zip——针对CO1基因标记的Miseq测序数据集，包含23个甲虫混合样本对应的46个fastq文件（1个样本测序失败）；
nfp4MBC.nf——用于处理Miseq数据集的Nextflow生物信息学脚本；
nextflow.config——使用nfp4MBC.nf时所需的配置文件；
adapters_16S.zip——用于标记24个甲虫混合样本16S基因的接头序列，同时为使用nfp4MBC.nf处理16S Miseq数据集时的必需文件；
adapters_CO1.zip——用于标记24个甲虫混合样本CO1基因的接头序列，同时为使用nfp4MBC.nf处理CO1 Miseq数据集时的必需文件；
rMBC.Rmd——用于群落分析的R Markdown代码文件；
rMBC.zip——rMBC.Rmd中使用的数据集；
COI_ZOTUs_176.fasta——176个COI ZOTU（Zero-radius Operational Taxonomic Unit）的DNA序列；
16S_ZOTUs_156——156个16S ZOTU的DNA序列；
提供机构：
University of Tasmania
创建时间：
2021-02-21
