20240903 Hamm & Gray spot plate images

E. coli strains MG1655, MJG0224 (MG1655 ∆ppk-749), MJG0226 (MG1655 ∆relA782), MJG0344 (MG1655 ∆rpoS746), MJG1116 (MG1655 ∆ppk-749 ∆relA782), MJG1119 (MG1655 ∆ppk-749 ∆rpoS746), MJG1136 (MG1655 ∆relA782 spoT207::cat+), MJG1137 (MG1655 ∆ppk-749 ∆relA782 spoT207::cat+), MJG1205 (MG1655 rpoB3449), MJG1234 (MG1655 ∆ppk-749 rpoB3449), MJG1235 (MG1655 ∆relA782 rpoB3449), MJG1236 (MG1655 ∆ppk-749 ∆relA782 rpoB3449), MJG1237 (MG1655 ∆relA782 spoT207::cat+ rpoB3449), MJG1241 (MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ rpoB3449), MJG1605 (MG1655 ∆relA782 spoT207::cat+ / pUC18 (bla+)), MJG1606 (MG1655 ∆relA782 spoT207::cat+ / pSPOT1 (spoT+, bla+)), MJG1608 (MG1655 ∆relA782 spoT207::cat+ / pSPOT3 (spoT D259N, bla+)), MJG1613 (MG1655 ∆relA782 spoT207::cat+ / pSPOT4 (spoT D73N D259N, bla+)), MJG1306 (MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ / pUC18 (bla+)), MJG1307 (MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ / pPPK8 (ppk+ bla+)), MJG1308 (MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ / pSPOT1 (spoT+ bla+)), MJG1609 (MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ / pSPOT2 (spoT D73N bla+)), MJG1610 (MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ / pSPOT3 (spoT D259N bla+)), or MJG1614 (MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ / pSPOT4 (spoT D73N D259N bla+)) were grown overnight at 37C with shaking in LB broth, then rinsed twice with PBS and normalized to A600 = 1.5-µl spots of 10-fold serial dilutions were plated on LB agar and on M9 agar containing 0.4% (w/v) glucose with or without 0.05% casamino acids (Fisher Scientific cat. #BP1424), 1% tryptone (Fisher Scientific cat. #BP1421), or 0.5% yeast extract (Becton Dickinson cat. #288620). Plates were incubated overnight at 30 or 37C, as indicated.

本数据集所使用的大肠杆菌（E. coli）菌株包括MG1655、MJG0224（MG1655 ∆ppk-749）、MJG0226（MG1655 ∆relA782）、MJG0344（MG1655 ∆rpoS746）、MJG1116（MG1655 ∆ppk-749 ∆relA782）、MJG1119（MG1655 ∆ppk-749 ∆rpoS746）、MJG1136（MG1655 ∆relA782 spoT207::cat+）、MJG1137（MG1655 ∆ppk-749 ∆relA782 spoT207::cat+）、MJG1205（MG1655 rpoB3449）、MJG1234（MG1655 ∆ppk-749 rpoB3449）、MJG1235（MG1655 ∆relA782 rpoB3449）、MJG1236（MG1655 ∆ppk-749 ∆relA782 rpoB3449）、MJG1237（MG1655 ∆relA782 spoT207::cat+ rpoB3449）、MJG1241（MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ rpoB3449）、MJG1605（MG1655 ∆relA782 spoT207::cat+ / pUC18 (bla+)）、MJG1606（MG1655 ∆relA782 spoT207::cat+ / pSPOT1 (spoT+, bla+)）、MJG1608（MG1655 ∆relA782 spoT207::cat+ / pSPOT3 (spoT D259N, bla+)）、MJG1613（MG1655 ∆relA782 spoT207::cat+ / pSPOT4 (spoT D73N D259N, bla+)）、MJG1306（MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ / pUC18 (bla+)）、MJG1307（MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ / pPPK8 (ppk+ bla+)）、MJG1308（MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ / pSPOT1 (spoT+ bla+)）、MJG1609（MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ / pSPOT2 (spoT D73N bla+)）、MJG1610（MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ / pSPOT3 (spoT D259N bla+)）以及MJG1614（MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ / pSPOT4 (spoT D73N D259N bla+)）。所有菌株均于37℃、振荡培养条件下在LB肉汤中过夜培养，随后用磷酸盐缓冲液（PBS）冲洗两次，并将菌液浓度调整至A600=1.5。取1.5 μL的10倍系列稀释菌液点样于LB琼脂平板，以及添加有0.4%（w/v）葡萄糖的M9琼脂平板；该M9琼脂平板可额外添加或不添加0.05%酪蛋白氨基酸（Fisher Scientific货号BP1424）、1%胰蛋白胨（Fisher Scientific货号BP1421）或0.5%酵母提取物（Becton Dickinson货号288620）。平板将按照实验设计置于30℃或37℃过夜培养。