Whole-genome sequencing of Kazachstania pintopolesii isolates from mice

Mouse feces and cecal content were plated on Sabouraud Dextrose Agar. Single colonies were re-streaked and then expanded for sequencing and cryopreservation. Genomic DNA was extracted from colonies using the QiaAmp DNA Mini Kit (Qiagen). Quality control, library preparation, and deep sequencing on Illumina HiSeqX platform were performed at Novogene Co., Ltd.

将小鼠粪便与盲肠内容物涂布接种于沙氏葡萄糖琼脂（Sabouraud Dextrose Agar）平板。挑取单菌落进行划线纯化后扩大培养，用于后续测序与低温保藏。采用QIAamp DNA迷你试剂盒（QiaAmp DNA Mini Kit, Qiagen）从菌落中提取基因组DNA。质量控制、文库制备及基于Illumina HiSeqX平台的深度测序均由诺禾致源（Novogene Co., Ltd.）完成。