Additional file 8: of Enrichment of extracellular vesicles from tissues of the central nervous system by PROSPR

Dataset 7. List of proteins identified in PROSPR-CNS-EVs from mouse brain. Database searches was performed using an in-house Mascot server (version 2.3.02, Matrix Science, MA, USA) with a precursor ion tolerance of 10 ppm and fragment peak tolerance of 0.05 Da. Uniprot mouse database (downloaded on 19th February of 2016, 101382 sequences and 43.874.034 residues) was used for database search. Two missed trypsin cleavage sites were tolerated. Carbamidomethylation (C) was set as fixed modification and deamidation (NQ) and oxidation (M) were set as variable modifications. Peptides with Mascot score >15 were used to generate the peptide list in all tested human conditions. (XLS 127 kb)

数据集7：从小鼠大脑PROSPR-CNS细胞外囊泡（PROSPR-CNS-EVs）中鉴定得到的蛋白质列表。数据库检索采用自研的Mascot服务器（版本2.3.02，Matrix Science公司，美国马萨诸塞州），设置前体离子耐受误差为10 ppm，碎片峰耐受误差为0.05 Da。本次检索使用的小鼠UniProt数据库于2016年2月19日下载，共计包含101382条序列与43874034个残基。实验允许最多2个胰蛋白酶酶切位点漏切，将半胱氨酸（C）的氨基甲酰化设为固定修饰，天冬酰胺/谷氨酰胺（NQ）的脱酰胺化以及甲硫氨酸（M）的氧化设为可变修饰。在所有测试的人类样本条件下，均选取Mascot得分大于15的肽段以构建肽段列表。（文件格式：XLS，大小127 kb）