Quantification of microbial cells and virus-like particles in the water column above Kermadec Trench

Water samples were collected using 7.5-Liter Niskin bottles and fixed with 25% electron microscopy graded glutaraldehyde (1% final concentration) and stored at -80°C until quantification by flow cytometry. Samples were measured in triplicates using a BD FACSCanto™ II flow cytometer, after staining with SYBR Green I (Brussaard 2004). The flow rate was 5–7 μl/min, as determined by BD Trucount™ Beads. The laser settings and gating examples can be found in the Supporting Information of Schauberger et al. (2021).

水样采用7.5升尼斯金采样瓶（Niskin bottles）采集，经25%电子显微镜级戊二醛固定（终浓度1%）后，于-80℃保存至流式细胞术（flow cytometry）定量分析。样本经SYBR Green I染色（Brussaard 2004）后，使用BD FACSCanto™ II流式细胞仪进行三次重复测量。流速为5–7 μl/min，由BD Trucount™ Beads测定。激光设置及门控示例详见Schauberger等人（2021）的支持信息。