Impact of aging on intestinal epithelial cells and reversible aspects by metformin or rapamycin treatment. Impact of aging on intestinal epithelial cells and reversible aspects by metformin or rapamycin treatment

Aging is a risk factor that initiates pathogenesis in the intestinal mucosa. We used single cell RNA sequencing to dissect the aging process and how effectively geroprotectors delays the process. Overall design: Intestinal epithelial cells from CB6F1 male mice were isolated by FACS (Epcam+, CD45 neg) at either 5-month-old (Young), or 24-month-old (Old). For treatment group, metformin or rapamycin treatment for old mice began at 21-month-old and sacrificed at 24-month-old.

衰老是可诱发肠道黏膜发病进程的风险因素。本研究采用单细胞RNA测序（single cell RNA sequencing）技术，解析衰老过程及抗衰老剂（geroprotectors）延缓该进程的效能。实验总体设计：分别从5月龄（青年组，Young）与24月龄（老年组，Old）的CB6F1雄性小鼠中，通过荧光激活细胞分选术（FACS, Fluorescence Activated Cell Sorting）分离得到Epcam阳性、CD45阴性的肠道上皮细胞。给药组老年小鼠于21月龄起接受二甲双胍（metformin）或雷帕霉素（rapamycin）给药，并于24月龄时处死。