AlkB-facilitated RNA methylation sequencing (ARM-seq) and Demethylase-thermostable group II intron RT tRNA sequencing (DM-tRNA-seq) [brain]

The goal of this study was to determine the state of the tRNA transcriptome in Brain tissue of Mus Musculus (Mouse). This includes expression data of mature tRNAs and tRNA fragments (tRFs), as well as associated modification states of these RNAs assessed using mismatching percentages from sequencing data. Included are ARM-seq and DM-tRNA-seq libraries derived from isolated total small RNA from C57BL/6J male brains. These experiments were performed in triplicate (biological) and subjected to NGS.

本研究旨在明确小家鼠（Mus Musculus，即小鼠）脑组织中的转运RNA（transfer RNA, tRNA）转录组状态。本数据集涵盖成熟tRNA及其片段（tRFs）的表达数据，同时包含基于测序数据错配百分比评估得到的上述RNA的相关修饰状态。本数据集包含从C57BL/6J品系雄性小鼠脑组织中分离得到的总小RNA所构建的ARM-seq与DM-tRNA-seq测序文库。本实验设置3次生物学重复，并进行了下一代测序（Next Generation Sequencing, NGS）。