miRNA profiling of serum, liver and white adipose tissues

Male C57BL/6J mice were housed in cages and maintained on a 12-hour light-dark cycle. STD (NMF; Oriental Yeast) and HFHS chow (D12331; Research Diet, New Brunswick, NJ) were used, the mice were sacrificed at 20 weeks of age. Total RNAs from serum, liver and epididymal fat were subjected to Illumina TruSeq Small RNA preparation and sequencing.将雄性C57BL/6J小鼠饲养于笼具中，维持12小时明暗交替光照周期。本实验使用标准饲料（STD, NMF；Oriental Yeast）与高脂高糖饲料（HFHS chow, D12331；Research Diet，美国新泽西州新不伦瑞克），于小鼠20周龄时实施安乐死。采集血清、肝脏及附睾脂肪组织的总RNA，采用Illumina TruSeq Small RNA文库制备方案完成建库并进行测序。