Small RNA_Seq of bursae from chickens vaccinated with MD vaccines and challenged with vv+MDV

Differentially expressed microRNAs were identified in bursa samples of a Marek’s disease (MD) resistant line and a susceptible line of birds in response to MD vaccine HVT or CVI/988 Rispens inoculation on the day of hatch and vv+MDV challenge 5 days post hatch. Total RNA samples were extracted from the bursa samples taken 21 days post the MDV challenge. Those microRNAs might be associated with both resistance to MD and protective efficacy of the MD vaccines.

本研究在马立克氏病（Marek’s disease, MD）抗性家禽品系与易感家禽品系的法氏囊样本中鉴定出差异表达的微小RNA（microRNAs）；供试家禽于出壳当日接种马立克氏病疫苗HVT或CVI/988 Rispens，并于出壳后5天攻毒超强毒马立克氏病病毒（vv+MDV）。研究人员于马立克氏病病毒（MDV）攻毒后21天采集上述法氏囊样本并提取总RNA。该类微小RNA或同时与马立克氏病的抗病性及马立克氏病疫苗的保护效力存在关联。