Transcriptional cycle suited to daytime N2 fixation in the unicellular cyanobacterium Candidatus Atelocyanobacterium thalassa (UCYN-A)

Investigation of whole genome gene expression level changes in two strains of the cyanobacteria Atelocyanobacterium thalasaa (UCYN-A) from environmental samples. The diel gene expression analyzed in this study is further described in Muñoz-Marin, M., I. N. Shilova, T. Shi, H. Farnelid & J. P. Zehr. 2017. Unicellular cyanobacterial symbiosis facilitates aerobic nitrogen fixation. Science (to be submitted). Two 8x60K array slides and one 4x180K array slide using total RNA extracted from seawater at the Scripps Institution of Oceanography (SIO) Ellen Browning Scripps Memorial Pier in La Jolla, CA between 28th July and 1st August 2014 during diel cycles. Each chip measures the expression level of 1194 genes from UCYN-A1 and 1244 genes from UCYN-A2 with up to six 60-mer probes per gene.

本研究针对环境样本中的两株海生远蓝杆菌（Atelocyanobacterium thalasaa，简称UCYN-A）的全基因组基因表达水平变化展开探究。本研究所分析的昼夜节律基因表达特征，已在Muñoz-Marin M.、Shilova I. N.、Shi T.、Farnelid H.与Zehr J. P.于2017年完成的《单细胞蓝藻共生体促进好氧固氮》（已提交至《Science》期刊）一文中进行了详细阐述。
实验于2014年7月28日至8月1日期间，在加利福尼亚州拉霍亚市斯克里普斯海洋研究所（Scripps Institution of Oceanography, SIO）艾伦·勃朗宁·斯克里普斯纪念码头采集海水样本，提取总RNA后，采用2张8×60K基因芯片与1张4×180K基因芯片完成检测，实验周期覆盖昼夜节律循环。
每张芯片可同时检测UCYN-A1的1194个基因与UCYN-A2的1244个基因的表达水平，每个基因配备至多6条60聚体探针。