Whole Genome Sequence and Molecular Characterization of phiCD146, a Siphoviridae Phage Infecting Clostridium difficile. Clostridioides difficile strain:phiCD146

Clostridium difficile strains have been investigated for the presence of functional prophages. Following mitomycine C induction, temperate phages have been isolated. Transmission electron microscopy (TEM) has revealed member of Siphoviridae / Myoviridae family. Phage DNA has been purified following phage amplification on sensitive host and subjected to complete genome sequencing using Illumina technology. Following sequencing, double stranded-DNA (dsDNA) genomes have been assembled and annotated.

本研究针对艰难梭菌（Clostridium difficile）菌株的功能性前噬菌体存在情况展开了探究。经丝裂霉素C（mitomycine C）诱导处理后，成功分离得到温和噬菌体。借助透射电子显微镜（Transmission Electron Microscopy, TEM）观测，确认分离得到的噬菌体隶属于长尾噬菌体科（Siphoviridae）与肌尾噬菌体科（Myoviridae）。通过在敏感宿主菌中扩增噬菌体，纯化获得噬菌体DNA，并采用Illumina测序技术完成全基因组测序。测序完成后，对双链DNA（double-stranded DNA, dsDNA）基因组进行了组装与注释。