Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - pool88_h1930002_A38. Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - pool88_h1930002_A38

Illustrating the cellular architecture of the human brain is critical to understanding its diverse functions and complex animal behaviors. Single nucleus methylation sequencing was applied to regions of human brains. we identified distinct cell clusters and formed them in a hierarchical taxonomy. These clusters include known primary brain cell types and possible sub-types. We use these data to identify the epigenomic characteristics and to define specific regulatory elements for each cell cluster. Overall design: Apply snmC-seq3 to brain regions to identify epigenomic landcape of the adult human brains

阐明人类大脑的细胞结构，对于理解其多样的功能与复杂的动物行为至关重要。本研究对人类大脑多个区域应用单细胞核甲基化测序（Single nucleus methylation sequencing）技术，鉴定得到多组独特的细胞簇并构建了层级分类系统。这些细胞簇涵盖了已被报道的主要脑细胞类型以及潜在的细胞亚型。本研究利用该数据集解析各组细胞簇的表观基因组特征，并明确了每个细胞簇特有的调控元件。整体实验设计：对大脑区域应用snmC-seq3技术，以解析成年人类大脑的表观基因组图谱。