T cell receptor spike-in detection power

Goal: To develop a combined computational and experimental pipeline for quantifying the detection power of bulk TCR sequencing approaches, based on a set of 45 unique spike-in TCRs within a wide frequency range (5x10-2 to 10-6).Study "T-Cell Receptor Detection Power of Bulk Sequencing for Immune Diagnostics: Quantification with a Panel of Spike-in Sequences and a Novel Power Calculator".

研究目标：开发一套计算与实验一体化的研究流程，用于量化批量T细胞受体（TCR）测序方法的检测效能。该流程基于一组覆盖宽相对丰度范围（5×10⁻² 至 10⁻⁶）的45种独特掺入型T细胞受体（spike-in TCRs）。本研究的正式名称为《用于免疫诊断的批量测序T细胞受体检测效能：基于掺入序列面板与新型效能计算器的量化分析》。