Raw Nanopore sequencing data of CD-pore-seq V1: TelN PromethION SRICO_PromeR2 1
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=Raw Nanopore sequencing data of CD-pore-seq V1= ==Author==Zicheng Yu1, Zihao Chen1, Thomas C. Williams1,2, Craig J. Anderson1,3,4, Andrew P. Jackson1,*, Martin A.M. Reijns1,*, Martin S. Taylor1,*1 MRC Human Genetics Unit, Institute of Genetics and Cancer, University of Edinburgh, UK.2 Child Life and Health, University of Edinburgh, UK.3 German Cancer Research Center, Heidelberg, Germany4 Department of Translational Oncology, German Cancer Research Center, Munich, Germany. * Correspondence should be addressed to andrew.jackson@ed.ac.uk, martin.reijns@ed.ac.uk and martin.taylor@ed.ac.uk ==Citation=='Generalised training for DNA modification detection by nanopore sequencing' ==Description==Nanopore sequencing data of libraries to train machine learning models to predict single DNA-embedded ribonucleotides.Ligation kit: LSK-114 (all except for ygDNA) or SQK-NBD114.96 (ygDNA)Flow-cell version: R10.4.1Sequencing device: MinION or PromethION (./TelN/PromethION only) ==TelN==* Sequencing the TelN library (high-complexity randomers surrounding single DNA-embedded ribonucleotides) on a MinION flow-cell (./MinION) or two PromethION flow-cells (PromethION), prepared by CD-pore-seq (with the second strand synthesised by the primer containing TelN recognition sequence, cut and covalently linked by TelN digestion).
=CD-pore-seq V1原始纳米孔测序数据= ==作者== Zicheng Yu¹, 陈子浩¹, Thomas C. Williams¹,², Craig J. Anderson¹,³,⁴, Andrew P. Jackson¹,*, Martin A.M. Reijns¹,*, Martin S. Taylor¹,* ¹ 英国爱丁堡大学遗传学与癌症研究所医学研究理事会人类遗传学单元; ² 英国爱丁堡大学儿童生命与健康系; ³ 德国海德堡德国癌症研究中心; ⁴ 德国慕尼黑德国癌症研究中心转化肿瘤学系。 * 通讯作者请联系:andrew.jackson@ed.ac.uk、martin.reijns@ed.ac.uk及martin.taylor@ed.ac.uk ==引用文献== 《基于纳米孔测序的DNA修饰检测通用训练方法》 ==数据集描述== 本数据集为用于训练机器学习模型以预测单DNA嵌入核糖核苷酸的文库纳米孔测序数据。所用连接试剂盒:LSK-114(ygDNA文库除外)或SQK-NBD114.96(仅ygDNA文库);测序流动槽(flow-cell)版本:R10.4.1;测序设备:MinION或PromethION(./TelN/PromethION 专用)。 ==TelN文库测序说明== * 本批次测序的TelN文库通过CD-pore-seq技术制备,包含环绕单DNA嵌入核糖核苷酸的高复杂度随机序列,可采用MinION流动槽(./MinION)或两台PromethION流动槽(PromethION)完成测序;该文库的制备流程为:通过携带TelN识别序列的引物合成第二链,经TelN酶切后进行共价连接。



