CIL:45865, Homo sapiens. In Cell Image Library

Plate 25571 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules.

Images
The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the .cell-painting. assay).

This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format.

编号25571的培养板——表型谱分析（Phenotypic profiling）旨在通过多参数、基于特征的分析手段，对每个样本的细胞表型进行系统性总结，使谱间的相似性能够反映样本之间的实际相似性。本图像集为测试基于图像的谱分析方法（image-based profiling methods）区分小分子效应的能力提供了基准支撑。
图像说明
本图像集中的成像样本为经1600种已知生物活性化合物分别处理的U2OS细胞，通过六种标记物完成七个细胞器的染色标记，该实验为细胞画描检测法（cell-painting assay）。
该预实验共包含20块培养板。每块板设有384个孔，每个孔采集9个成像视野，总计69120个成像视野。每个视野通过5个检测波长通道完成成像，每个通道均保存为独立的灰度图像文件，因此该数据集共包含345600张16位TIFF格式的图像文件。