DNA Methylome Profiling of Granulosa Cells Reveals Altered Methylation in Genes Vital to Ovarian Functions in PCOS

These are the supplemental file-sets for the DNA methylome profiling data of ovarian granulosa cells between controls and women with PCOS. The data was generated using a high throughput methyl-capture sequencing (MC-seq) approach and few of the identified differentially methylated genes (DMGs), which are vital for normal functioning of the ovary were validated using pyrosequencing and real-time PCR. Validation of genes such as AKR1C3, CASR, GHRHR, RETN, MAMLD1, TNF and TF provided novel insights on the involvement of epigenetic modifications in regulation of processes such as folliculogenesis, angiogenesis, steroidogenesis, luteal maintenance, ovulation, oocyte maturation, oocyte developmental competence and many more, which are compromised in women with PCOS. This data also generated valuable information on pathways and biological processes playing crucial roles in the pathophysiology of PCOS.

本数据集为对照组与多囊卵巢综合征（Polycystic Ovary Syndrome, PCOS）女性卵巢颗粒细胞的DNA甲基化组谱分析数据的补充文件集。该数据采用高通量甲基化捕获测序（methyl-capture sequencing, MC-seq）技术生成，针对少量经鉴定的、对卵巢正常功能至关重要的差异甲基化基因（differentially methylated genes, DMGs），通过焦磷酸测序与实时荧光定量PCR进行了验证。对AKR1C3、CASR、GHRHR、RETN、MAMLD1、TNF及TF等基因的验证，为表观遗传修饰参与调控卵泡发生、血管生成、类固醇生成、黄体维持、排卵、卵母细胞成熟、卵母细胞发育能力等诸多在多囊卵巢综合征女性中受损的生物学过程提供了全新见解。本数据集还为阐明在多囊卵巢综合征病理生理过程中发挥关键作用的信号通路与生物学过程提供了宝贵信息。