Assessment of transcriptional ERa activity following exposure to 17a-E2 and 17b-E2

The study shows that, regardless of dose, both estrogens elicit nearly identical ERa activity Overall design: U2OS Cells were transfected with ERa and were exposed to vehicle, 17a-E2 (10nM & 100nM), or 17b-E2 (10nM) for 24 hours in preparation for RNA and ChIP sequencing analyses

该研究表明，无论剂量如何，两种雌激素均可诱导几乎一致的ERα（Estrogen Receptor α）活性。实验设计：将ERα（Estrogen Receptor α）转染至U2OS细胞中，分别以溶剂对照、10纳摩尔与100纳摩尔的17α-雌二醇（17α-E2）、以及10纳摩尔的17β-雌二醇（17β-E2）处理细胞24小时，以开展后续RNA测序与染色质免疫沉淀测序（ChIP Sequencing）分析。