GRADE evaluation for inflammation factors.

Objective
This study aimed to assess the correlation between the circulating cell-free mitochondria DNA and inflammation factors in noninfectious disease by meta-analysis of data from eligible studies.
Materials and methods
Through a comprehensive searching of pubmed, embase, web of science, cochrane from establishment of the database to October 31, 2022, studies were selected that investigated the association of circulating cell free mitochondria DNA with inflammatory factors in non-infectious diseases. Studies that met the inclusion criteria and were published in English or Chinese were included. Data of each correlation coefficients were extracted from the paper and 95% confidence intervals were calculated. Sensitivity and heterogeneity tests were carried out for each data.
Results
A total of 660 articles were retrieved and 22 were included in this meta-analysis, including 2600 patients. A fixed effects model was employed to examine ISS and IL-8, others were analyzed using random effects models. The correlation coefficient between mtDNA and ISS score was 0.37 (95%CI = [0.232;0.494]), p<0.0001, heterogeneity I2 = 46%, p = 0.11). The correlation coefficients between mtDNA and inflammatory factors are as follows: TNFα, 0.405 [(95%CI = [0.253;0.538], p<0.0001, heterogeneity I2 = 77%, p = 0.0001]. IL-6, 0.469 [(95%CI = [0.296;0.612]), p = 0.0001, heterogeneity I2 = 93%, p<0.0001]. CRP, 0.333[(95%CI = [0.149;0.494]), p = 0.005, heterogeneity I2 = 85%, p<0.0001]. IL-8, 0.343[(95%CI = [0.233;0.524]), p = 0.001, heterogeneity I2 = 50%, p = 0.09]. PCT, 0.333 [(95%CI = [0.06;0.64]), p = 0.09,heterogeneity I2 = 64%,p = 0.06]. There were no significant publication bias for TNFα, IL-6 and CRP.
Conslusion
Circulating cell free mtDNA was moderate positively correlated with the expression of inflammatory factors and the degree of trauma.

研究目的
本研究旨在通过对符合纳入标准的研究数据进行荟萃分析，评估非感染性疾病患者外周血循环游离线粒体DNA（circulating cell-free mitochondria DNA）与炎症因子的相关性。

材料与方法
本研究于2022年10月31日前，对PubMed、Embase、Web of Science、Cochrane数据库自建库起的相关文献开展全面检索，筛选探讨非感染性疾病患者循环游离线粒体DNA与炎症因子关联的研究。纳入符合纳入标准且以英文或中文发表的文献。从纳入文献中提取各相关系数，并计算95%置信区间（95% confidence intervals, 95%CI）。对所有纳入数据进行敏感性分析与异质性检验。

结果
本研究共检索到660篇文献，最终纳入22篇进行本次荟萃分析，涉及患者2600例。针对ISS与IL-8采用固定效应模型进行分析，其余指标均采用随机效应模型。线粒体DNA与ISS评分的相关系数为0.37（95%CI=[0.232;0.494]），p<0.0001，异质性I²=46%，p=0.11。线粒体DNA与各炎症因子的相关系数如下：TNF-α，0.405（95%CI=[0.253;0.538]，p<0.0001，异质性I²=77%，p=0.0001）；IL-6，0.469（95%CI=[0.296;0.612]，p=0.0001，异质性I²=93%，p<0.0001）；CRP，0.333（95%CI=[0.149;0.494]，p=0.005，异质性I²=85%，p<0.0001）；IL-8，0.343（95%CI=[0.233;0.524]，p=0.001，异质性I²=50%，p=0.09）；PCT，0.333（95%CI=[0.06;0.64]，p=0.09，异质性I²=64%，p=0.06）。TNF-α、IL-6及CRP无显著发表偏倚。

结论
循环游离线粒体DNA与炎症因子表达水平及创伤程度呈中度正相关。