Profiling of expression level in adult female Nasonia vitripennis. Nasonia vitripennis

The expression level in the parasitoid Nasonia vitripennis adult female samples was profiled and compared with the methylation pattern. Methylated and non-methylated genes showed markedly different patterns. The expression level was higher for methylated than non-methylated genes. non-nmethylated genes account for 99% of the genes that were not found to be expressed in the adult female RNA-seq data (FPKM < 0.1). Unlike methylated genes, the expression distribution of the non-methylated genes was bimodal with a lower expressed group and a moderately expressed group of genes, indicating that methylation status is not the only determinant of high expression in adult females. Methylated genes also have lower coefficient of variation (CV) of expression level across five developmental stages, suggesting that they are expressed more evenly across development. Overall design: Profiling of expression level in adult female Nasonia vitripennis by RNA-seq

本研究对寄生蜂丽蝇蛹集金小蜂（Nasonia vitripennis）成虫雌虫样本的基因表达水平进行了检测，并与甲基化模式开展对比分析。结果表明，甲基化基因与非甲基化基因呈现出显著不同的表达特征：甲基化基因的表达水平高于非甲基化基因。在成虫雌虫RNA测序（RNA-seq）数据中未检测到表达（FPKM < 0.1）的基因中，非甲基化基因占比达99%。与甲基化基因不同，非甲基化基因的表达分布呈双峰模式，可分为低表达组与中度表达组，这提示甲基化状态并非成虫雌虫基因高表达的唯一决定因素。此外，甲基化基因在5个发育阶段的表达水平变异系数（CV）更低，说明其在整个发育进程中的表达更为均匀稳定。实验整体设计：通过RNA测序（RNA-seq）检测丽蝇蛹集金小蜂成虫雌虫的基因表达水平。