Glycoproteome of Elongating cotton fiber cells

Glycoproteins isolated from elongating cotton fiber cells were analyzed using 2D-PAGE followed by MALDI TOF/TOF based protein identification. Protein isoforms were identified using Tandem mass spectrometry. Lectin based Glycopeptide enrichment strategy followed by PNGase catalyzed deglycosylation reaction was employed to assign potential N-linked glycosylation sites.

从伸长期棉纤维细胞中分离得到的糖蛋白（Glycoprotein），经双向凝胶电泳（2D-PAGE）分离后，采用基于基质辅助激光解吸电离飞行时间/飞行时间质谱（MALDI TOF/TOF）的蛋白质鉴定方法进行分析。利用串联质谱（Tandem mass spectrometry）鉴定蛋白质同工型，并通过基于凝集素（Lectin）的糖肽富集策略结合肽N-糖苷酶（PNGase）催化的去糖基化反应，推定潜在的N-连接糖基化位点。