RNAseq of bulk regulatory T cells (Tregs) and conventional T cells (Tconvs) during acute kidney injury and fibrosis development

We performed bulk RNA-seq from FACS-sorted Tregs (Foxp3+) and T convs (Foxp3-) CD4+ T cells from kidneys and spleens at different phases after injury.我们针对损伤后不同阶段的肾脏与脾脏组织，提取经荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）获得的CD4+ T细胞亚群——叉头框蛋白P3阳性（Foxp3+）调节性T细胞（Tregs）与叉头框蛋白P3阴性（Foxp3-）常规CD4+ T细胞（T convs），并对其开展批量RNA测序（bulk RNA-seq）。