A Novel Synergistic Action of phage vB_PaeP_GZMU_A1002 with Allicin against Carbapenem-resistant Pseudomonas aeruginosa

The increasing problem of bacterial resistance to antibiotics necessitates the exploration of innovative antimicrobial strategies. Phage therapy is a promising solution; however, naturally occurring phages targeting resistant pathogens remain limited. This study aimed to isolate and identify phages that lyse carbapenem-resistant Pseudomonas aeruginosa (CRPA) from sewage and investigate the synergistic antimicrobial effects of phage therapy combined with allicin. A novel phage, vB_PaeP_GZMU_A1002, was successfully isolated and characterized, revealing a double-stranded DNA genome of 63,770 bp with no virulence or resistance genes, indicating its safety for application. This phage demonstrated good stability across various temperatures and pH levels, effectively inhibited the CRPA growth within 12 hours, and achieved a biofilm clearance rate of about 50%. Notably, the combination of phage and allicin significantly enhanced antimicrobial efficacy, reducing effective concentrations of both agents. The synergistic action was likely due to increased damage to bacterial cell membranes and reduced cell surface hydrophobicity. This research highlights the potential of phage therapy, particularly when combined with natural compounds like allicin, as a viable approach to combat antibiotic-resistant infections.

细菌对抗生素的耐药性问题日益严峻，亟需探索创新性的抗菌策略。噬菌体疗法是一种颇具前景的解决方案，但天然存在的靶向耐药致病菌的噬菌体仍较为稀缺。本研究旨在从污水中分离并鉴定能够裂解耐碳青霉烯类铜绿假单胞菌（carbapenem-resistant Pseudomonas aeruginosa, CRPA）的噬菌体，并探究噬菌体疗法与大蒜素联合使用的协同抗菌效果。本研究成功分离并鉴定了一株新型噬菌体vB_PaeP_GZMU_A1002，其基因组为63770 bp的双链DNA，未携带任何毒力或耐药基因，表明其应用安全性良好。该噬菌体在不同温度与pH条件下均表现出良好的稳定性，可在12小时内有效抑制CRPA的生长，并实现约50%的生物膜清除率。值得注意的是，噬菌体与大蒜素联合使用可显著提升抗菌效果，同时降低二者的有效使用浓度。其协同作用机制可能与细菌细胞膜损伤加剧以及细胞表面疏水性降低有关。本研究凸显了噬菌体疗法的应用潜力，尤其是与大蒜素这类天然化合物联合使用时，可为对抗耐药菌感染提供一种可行的解决方案。