Differential gene expression in HCC cell lines Huh7 and PLC under high and low glucose culturement

To gain a deeper understanding of the mechanism by lncRNAs involved in glucose metabolism during the progression of HCC, we conducted transcriptome sequencing on HCC cells treated with low (5 mM) and high (25 mM) glucose and carried out analyses for the lncRNAs with a significant change of expression combined with TCGA-LIHC data. Huh7 and PLC cells were treated with low glucose (5mM) or high glucose (25mM) for 48 h, whole-transcriptome sequencing (RNA-Seq) was performed to identify the significant changed lncRNAs with glucose. To investigate the role of glucose-related lncRNAs in HCC，we performed an RNA-sequencing analysis with Huh7 and PLC cells cultured in low glucose (5 mM) and high glucose (25 mM) for 48 h.Comparative gene expression profiling analysis of RNA-seq data for high glucose and low glucose treatment for 48 h in Huh7 and PLC cells.

为深入探究长链非编码RNA（lncRNAs）在肝细胞癌（Hepatocellular Carcinoma, HCC）进展过程中参与糖代谢的分子机制，我们对经低浓度（5 mM）与高浓度（25 mM）葡萄糖处理的肝癌细胞开展了转录组测序，并结合TCGA-LIHC数据集对表达存在显著差异的lncRNAs进行分析。我们将Huh7与PLC细胞置于低浓度（5 mM）或高浓度（25 mM）葡萄糖培养基中培养48小时，通过全转录组测序（RNA-Seq）筛选出受葡萄糖浓度调控的差异表达lncRNAs。为进一步明确糖代谢相关lncRNAs在HCC中的生物学功能，我们再次对经上述相同培养条件处理的Huh7和PLC细胞开展RNA测序分析。此外，我们针对高、低葡萄糖处理48小时后的Huh7与PLC细胞的RNA测序数据，开展了基因表达谱对比分析。