Analysis of virome by high-throughput sequencing disclosed multiple infection in a single grapevine plant

ABSTRACT: Among phytosanitary problems of the grapevine, viruses stand out for their capacity of reducing the quality and yield of grapes. However, detecting and identifying viral infections in grapevines can be challenging. This study performed a high throughput sequencing (HTS) of the viral pathogens present in a vine showing virus-like symptoms to elucidate the etiology. HTS analysis reported in a hybrid grapevine with mild curling down of leaf edges, the presence of four viruses and viroids, which were probably implicated in the observed symptoms. The determined complete genomes showed high genetic identities with previously characterized isolates of homologous pathogens.

摘要：在葡萄的各类植物检疫问题中，病毒因可降低葡萄品质与产量而最为突出。然而，在葡萄植株中检测并鉴定病毒感染仍颇具挑战。本研究对一株表现出类病毒症状的葡萄藤所携带的病毒病原体进行了高通量测序（high throughput sequencing，HTS），以阐明其病原成因。HTS分析结果显示，在一株表现为叶片边缘轻度下卷的杂交葡萄植株中，存在四种病毒与类病毒（viroids），这些病原大概率与观测到的症状相关。本研究测定的完整基因组与此前已完成表征的同源病原体分离株具有较高的遗传一致性。