Transcriptional profiling of Schneider cells to identify crc target genes after 3 hours expression

Transcriptional profiling of Schneider 2 cells comparing control stable inducible HA-expressing cells with stable inducible HA-crc expressing cells after 3h of induction with 0.7mM copper sulphate. Two-condition experiment, control cells stable inducible S2 HA.pMT-Puro cells vs. stable inducible S2 HA-crc.pMT-Puro cells after 6h induction with 0.7 mM copper sulphate. experiment was performed in three independent biological replicates.

本实验针对Schneider 2细胞（Schneider 2 cells）开展转录组谱分析，对比经0.7mM硫酸铜（copper sulphate）诱导3小时后，对照的稳定诱导型HA表达细胞与稳定诱导型HA-crc表达细胞的转录特征。本实验为双条件对照实验，以经0.7mM硫酸铜诱导6小时后的稳定诱导型S2 HA.pMT-Puro细胞作为对照组，与稳定诱导型S2 HA-crc.pMT-Puro细胞进行比较。本次实验共完成3次独立生物学重复（biological replicates）。