Main technical parameters of DMD.

Laser microdissection technology is favored by biomedical researchers for its ability to rapidly and accurately isolate target cells and tissues. However, the precision cutting capabilities of existing laser microdissection systems are hindered by limitations in overall mechanical movement accuracy, resulting in suboptimal cutting quality. Additionally, the use of current laser microdissection systems for target acquisition may lead to tissue burns and reduced acquisition rates due to inherent flaws in the capture methods. To address these challenges and achieve precise and efficient separation and capture of cellular tissues, we integrated a digital micromirror device (DMD) into the existing system optics to modulate spatial light. This allows the system to not only implement the traditional point scanning cutting method but also utilize the projection cutting method.We have successfully cut various patterns on commonly used laser microdissection materials such as PET films and mouse tissues. Under projection cutting mode, we were able to achieve precise cutting of special shapes with a diameter of 7.5 micrometers in a single pass, which improved cutting precision and efficiency. Furthermore, we employed a negative pressure adsorption method to efficiently collect target substances. This approach not only resulted in a single-pass capture rate exceeding 90% for targets of different sizes but also enabled simultaneous capture of multiple targets, overcoming the limitations of traditional single-target capture and enhancing target capture efficiency, and avoiding potential tissue damage from lasers.In summary, the integration of the digital micromirror device into laser microdissection systems significantly enhances cutting precision and efficiency, overcoming limitations of traditional systems. This advancement demonstrates the accuracy and effectiveness of laser microdissection systems in isolating and capturing biological tissues, highlighting their potential in medical applications.

激光显微切割技术（Laser microdissection technology）凭借可快速、精准分离目标细胞与组织的特性，深受生物医学研究者的青睐。然而，现有激光显微切割系统的精准切割能力受限于整体机械运动精度不足，导致切割质量欠佳。此外，当前激光显微切割系统所采用的捕获方法存在固有缺陷，在获取目标样本时可能造成组织灼伤并降低捕获效率。为解决上述难题，实现细胞组织的精准高效分离与捕获，本研究将数字微镜器件（Digital Micromirror Device, DMD）集成至现有系统的光学模块中以调制空间光，使得系统既可实现传统的单点扫描切割模式，亦可采用投影切割模式。本研究已在PET膜、小鼠组织等常用激光显微切割耗材上成功切割出多种图案。在投影切割模式下，单次扫描即可精准切割直径7.5微米的特殊形状，有效提升了切割精度与效率。此外，本研究采用负压吸附法高效收集目标物质，该方法不仅可实现不同尺寸目标的单次捕获率超90%，还能同时捕获多个目标，突破了传统单目标捕获的局限，提升了目标捕获效率，同时避免了激光对组织造成的潜在损伤。综上，将数字微镜器件集成至激光显微切割系统中，可显著提升切割精度与效率，突破传统系统的性能局限。该技术方案验证了激光显微切割系统在生物组织分离与捕获中的准确性与有效性，凸显了其在医学应用中的潜力。