SPOROS: A pipeline to analyze DISE/6mer Seed Toxicity

The SPOROS pipeline is a read-based sequence analysis pipeline for studying 6mer Seed Toxicity in mouse and human RNA-seq data. Ago protein associated short RNAs were pulled down using a GW182 peptide and subjected to smRNA Seq

SPOROS分析流程是一款基于测序读段的序列分析流程，用于在小鼠和人类的RNA测序（RNA-seq）数据中研究六聚体种子毒性（6mer Seed Toxicity）。研究人员使用GW182肽段免疫沉淀捕获了与Ago蛋白结合的短RNA，并将其进行小分子RNA测序（smRNA Seq）。