LC-MS/MS proteomic analysis of mouse brown adipose tissues

This project aims to identify the proteins present in RNA granules of brown adipose tissue upon exposure to cold challenge. Wild type C57BL/6J mice were born and raised at thermoneutrality and were then subjected to cold exposure for 90 minutes. Brown adipose tissues were collected, and the protein lysates of RNA granules isolated from the brown adipose tissues were separated using SDS-PAGE gels and visualized with Coomassie stain. The protein bands were subsequently excised for downstream sample processing.

本项目旨在鉴定冷刺激下棕色脂肪组织（brown adipose tissue）中RNA颗粒（RNA granules）内存在的蛋白质。野生型C57BL/6J小鼠在热中性条件下出生并饲养，随后接受90分钟的冷暴露处理。收集棕色脂肪组织后，对从该组织中分离得到的RNA颗粒的蛋白质裂解液采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）凝胶进行分离，并通过考马斯亮蓝染色（Coomassie stain）实现可视化。随后切取蛋白条带以进行下游样品处理。