Expression data of early skin development mice. Mus musculus

Mice with the spontaneous uncv mutation are hairless. We utilize the uncv null mouse to investigate the underlying mechanisms giving rise to the relevant phenotypes and reveal significant changes in certain signaling pathways by microarray. Keywords: RNA expression study Overall design: Dorsal skins were taken from 4-day-old uncv null mice and wild type mice (as control), and they are sisters-german. The parent is uncv heterozygosis(uncv +/-), and their children has 3 genotype: wild(uncv +/+), heterozygosis(uncv +/-) and null (uncv -/-). We can easily distinguish them by their beard length and shape or identify their genotype by PCR using specific primers. HR 02 is a wild mouse sample and uncv null mice expresses as HR 03 and HR 04. RNA isolation was performed with the QIAGEN RNeasy mini kit (QIAGEN, Valencia, CA, USA) following standard instructions.

携带自发uncv突变的小鼠表现为无毛表型。本研究利用uncv纯合敲除小鼠，探究该类表型产生的潜在分子机制，并通过基因芯片（microarray）分析揭示了部分信号通路的显著变化。关键词：RNA表达研究
总体实验设计：采集4日龄uncv纯合敲除小鼠与野生型小鼠（作为对照）的背部皮肤组织，二者为同胞姐妹。其亲本为uncv杂合子（uncv +/-），子代共有三种基因型：野生型（uncv +/+）、杂合型（uncv +/-）与纯合敲除型（uncv -/-）。可通过胡须的长度与形态进行初步区分，或使用特异性引物通过聚合酶链式反应（PCR）鉴定其基因型。HR 02为野生型小鼠样本，HR 03与HR 04为uncv纯合敲除小鼠样本。RNA提取采用QIAGEN RNeasy迷你试剂盒（QIAGEN，美国加利福尼亚州巴伦西亚市），严格遵循标准操作流程完成。