The Diverse Phenotypic and Mutational Landscape Induced by Fluoroquinolone Treatment. The Diverse Phenotypic and Mutational Landscape Induced by Fluoroquinolone Treatment

We conducted whole genome sequencing on eight evolved E. coli strains (S1–S8) and the parental wild-type (WT) strain to identify mutations arising from ofloxacin treatments. These strains (S1-S8), generated through fluoroquinolone-mediated adaptive laboratory evolution (ALE), exhibited varying levels of tolerance and resistance. The ALE experiment involved intermittent antibiotic treatments of eight independent cultures over 22 days. The untreated WT strain served as a baseline to pinpoint mutations in the evolved strains.

本研究对8株经演化的大肠杆菌（E. coli）菌株（S1至S8）以及亲本野生型（wild-type，WT）菌株开展全基因组测序（whole genome sequencing），以鉴定氧氟沙星（ofloxacin）处理诱导产生的突变。上述S1至S8菌株通过氟喹诺酮类（fluoroquinolone）介导的自适应实验室演化（adaptive laboratory evolution，ALE）构建得到，其对药物的耐受性与耐药性水平各不相同。本次自适应实验室演化实验为期22天，对8组独立培养物实施间歇性抗生素处理。未接受处理的野生型菌株作为对照基线，用于精准定位演化菌株中的突变位点。