Role of RB1 in neurodegenerative diseases: Inhibition of post-mitotic neuronal apoptosis via Kmt5b

To better characterize the population of proliferating and apoptotic neuron in sibling and zrb1-ko, we performed 10× single-cell RNA sequencing (scRNA-seq) on whole-brain cells of sibling and zrbi-ko at 3 dpf. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enriched analysis of differentially expressed genes (DEGs) in NSPCs, myelencephalon neurons or cerebellum neurons between sibling and zrb1-ko mutants was performed.

为更好地表征同窝野生型对照与zrb1基因敲除个体中的增殖与凋亡神经元群体，我们对受精后3天（days post fertilization，dpf）的同窝野生型对照与zrb1基因敲除个体的全脑细胞开展10×单细胞RNA测序（single-cell RNA sequencing，scRNA-seq）。随后，我们对神经干细胞/祖细胞（Neural Stem/Progenitor Cells，NSPCs）、延脑神经元及小脑神经元中，同窝野生型对照与zrb1基因敲除突变体间的差异表达基因（differentially expressed genes，DEGs）开展京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）通路富集分析。