Data from: Persistence of the Probiotic Lacticaseibacillus rhamnosus Strain GG (LGG) in an In Vitro Model of the Gut Microbiome

Using the SHIME (an in vitro simulator of the human gut microbiome) we tracked the fate of the probiotic Lacticaseibacillus rhamnosus GG (LGG) over time and across colonic regions. Using fecal inoculum from three healthy human donors, reactors were established representing three colonic regions and both the luminal and mucosal microbiome in those regions. Community composition before, during, and after inoculation of the reactors with LGG as well as short chain fatty acid concentrations representing microbiome metabolic outputs. This dataset includes short-chain fatty acid concentrations and qPCR-based cell concentrations. Raw 16S rRNA amplicon sequencing of the V1-V2 regions can be found in the NCBI Sequence Read Archive associated with BioProject PRJNA893635: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA893635.

Resources in this dataset:

Resource Title: Short Chain Fatty Acid concentrations
File Name: LGG_SCFA_data.csv

Resource Title: LGG qPCR concentrations
File Name: LGG_qPCR_data.csv

Resource Title: Sample metadata
File Name: LGG_16S_metadata.csv

本研究采用SHIME（人体肠道微生物组体外模拟器），追踪了益生菌鼠李糖乳杆菌GG（Lacticaseibacillus rhamnosus GG，LGG）在不同结肠区域内随时间推移的存活与定植命运。我们采集3名健康人类供体的粪便接种物，构建了可模拟结肠三个区域管腔与黏膜微生物组的反应器体系。我们同时记录了反应器接种LGG前、接种过程中及接种后的群落组成，以及反映微生物组代谢产出的短链脂肪酸浓度。
本数据集包含短链脂肪酸浓度与基于定量PCR（qPCR）的细胞浓度数据。针对V1-V2区域的原始16S rRNA扩增子测序数据，可在隶属于BioProject PRJNA893635的NCBI序列读取档案（Sequence Read Archive）中获取，链接为：https://www.ncbi.nlm.nih.gov/bioproject/PRJNA893635。
本数据集包含以下资源：
- 资源标题：短链脂肪酸浓度，文件名：LGG_SCFA_data.csv
- 资源标题：LGG定量PCR细胞浓度，文件名：LGG_qPCR_data.csv
- 资源标题：样本元数据，文件名：LGG_16S_metadata.csv