Adult grade II astrocytomas are genetically distinct from their pediatric counterparts, and display a more stem cell-like expression pattern linked with more aggressive behaviour

We have used microarray comparative genomic hybridization (aCGH) at 1Mb resolution to study copy number changes in a series of WHO Grade II Astrocytomas (n=21). We have used Illumina arrays to study genome-wide expression patterns in a series of WHO Grade II Astrocytomas (n=10). Keywords: Array Comparative Genomic Hybridization (aCGH), Expression microarray [aCGH]: Single hybridization per case. 21 astrocytomas WHO grade II were analyzed. Target (tumor) labelled with Cy5 and reference with Cy3. Mixture of 20 normal male or female genomic DNA was used in sex-mismatched hybridization. [mRNA]: Single hybridization per case. 10 astrocytomas WHO grade II were analyzed (5 adult, 5 pediatric)

本研究采用分辨率为1Mb的微阵列比较基因组杂交（microarray Comparative Genomic Hybridization, aCGH）技术，对一系列WHO II级星形细胞瘤（n=21）的拷贝数变异进行研究。同时采用Illumina芯片，对一系列WHO II级星形细胞瘤（n=10）的全基因组表达模式展开分析。
关键词：阵列比较基因组杂交（Array Comparative Genomic Hybridization, aCGH）、表达微阵列。
[aCGH]：每个病例单次杂交。共分析21例WHO II级星形细胞瘤，以Cy5标记靶标（肿瘤样本），以Cy3标记参照样本。实验采用20份正常男性或女性基因组DNA的混合样本开展性别错配杂交。
[mRNA]：每个病例单次杂交。共分析10例WHO II级星形细胞瘤（其中成人5例，儿童5例）