sequencing of yeast RNAi libraries for improved protein production

Yeast RNAi libraries, YeaLKD-lib and YeaHKD-lib, constructed based on plasmid with different DNA insert, was subject to microfluidic droplet screening to enrich variant cells with high amylase production. The enriched libraries, post_sorted_YeaLKD-lib and post_sorted_YeaHKD-lib as well as YeaLKD-lib and YeaHKD-lib were then used for plasmid extraction and amplicon sequencing for the inserts respectively.

基于携带不同DNA插入片段的质粒构建的酵母RNA干扰文库YeaLKD-lib与YeaHKD-lib，经微流控液滴筛选(microfluidic droplet screening)以富集高产淀粉酶的变异细胞。富集得到的文库包括分选后YeaLKD-lib（post_sorted_YeaLKD-lib）、分选后YeaHKD-lib（post_sorted_YeaHKD-lib）以及原始的YeaLKD-lib与YeaHKD-lib；随后分别针对各文库开展质粒提取及对应插入片段的扩增子测序(amplicon sequencing)。