human adenovirus-host protein interactions

As intracellular parasites, viruses exploit cellular proteins at every stage of infection. Adenovirus related outbreaks cause severe acute respiratory illnesses and conjunctivitis, with no specific antiviral therapy yet available. Herein, we investigate human adenovirus (HAdV-D37) pIIIa-host protein interactions by affinity purification and mass spectrometry (AP-MS) screens. We demonstrate that viral pIIIa interacts with ubiquitin-specific protease 9x (USP9x) and Ran-binding protein 2 (RANBP2). As intracellular parasites, viruses exploit cellular proteins at every stage of infection. Adenovirus related outbreaks cause severe acute respiratory illnesses and conjunctivitis, with no specific antiviral therapy yet available. Herein, we investigate human adenovirus (HAdV-D37) pIIIa-host protein interactions by affinity purification and mass spectrometry (AP-MS) screens. We demonstrate that viral pIIIa interacts with ubiquitin-specific protease 9x (USP9x) and Ran-binding protein 2 (RANBP2).

作为胞内寄生生物，病毒在感染的各个阶段均会利用宿主细胞蛋白。腺病毒感染暴发可引发重症急性呼吸道疾病与结膜炎，目前尚无特异性抗病毒治疗手段。本研究通过亲和纯化-质谱（AP-MS）筛选技术，对人腺病毒（HAdV-D37）pIIIa与宿主蛋白的相互作用展开研究。本研究证实，病毒pIIIa可与泛素特异性蛋白酶9x（USP9x）以及Ran结合蛋白2（RANBP2）发生相互作用。
作为胞内寄生生物，病毒在感染的各个阶段均会利用宿主细胞蛋白。腺病毒感染暴发可引发重症急性呼吸道疾病与结膜炎，目前尚无特异性抗病毒治疗手段。本研究通过亲和纯化-质谱（AP-MS）筛选技术，对人腺病毒（HAdV-D37）pIIIa与宿主蛋白的相互作用展开研究。本研究证实，病毒pIIIa可与泛素特异性蛋白酶9x（USP9x）以及Ran结合蛋白2（RANBP2）发生相互作用。