QuaCRS

QuaCRS (Quality Control for RNA-Seq) is an integrated, simplified quality control (QC) system for RNA-seq data that allows easy execution of several open-source QC tools, aggregation of their output, and the ability to quickly identify quality issues by performing meta-analyses on QC metrics across large numbers of samples in different studies. It comprises two main sections. First is the QC Pack wrapper, which executes three QC tools: FastQC, RNA-SeQC, and selected functions from RSeQC. Combining these three tools into one wrapper provides increased ease of use and provides a much more complete view of sample data quality than any individual tool. Second is the QC database, which displays the resulting metrics in a user-friendly web interface. It was designed to allow users with less computational experience to easily generate and view QC information for their data, to investigate individual samples and aggregate reports of sample groups, and to sort and search samples based on quality. The structure of the QuaCRS database is designed to enable expansion with additional tools and metrics in the future.

QuaCRS（RNA测序质量控制，Quality Control for RNA-Seq）是一款集成化、轻量化的RNA测序数据质量控制（QC）系统，可便捷运行多款开源QC工具，整合其输出结果，并能通过对不同研究中大量样本的QC指标开展元分析，快速定位质量问题。该系统包含两大核心模块：其一为QC工具包封装模块，可运行三款QC工具：FastQC、RNA-SeQC以及RSeQC的选定功能。将这三款工具整合至单一封装中，不仅提升了易用性，还能比单一工具更全面地展现样本数据的质量全貌。其二为QC数据库模块，可通过用户友好的网页界面展示生成的各项质量指标。该系统的设计初衷是让计算经验不足的用户能够轻松为其数据生成并查看QC信息，可对单个样本进行分析、汇总样本组的检测报告，并基于质量指标对样本进行排序与检索。QuaCRS数据库的架构设计预留了扩展空间，未来可接入更多QC工具与质量指标。