Effects of dexamethasone on human skeletal muscle and adipose tissue. Homo sapiens

Context: Glucocorticoids are frequently prescribed drugs with important side-effects such as glucose intolerance and tissue remodelling. Objective: The goal was to explore the molecular basis of the crosstalk between adipose tissue and skeletal muscle during short term glucocorticoid treatment. Design and Intervention: Healthy male subjects were assigned to a 4-d treatment with dexamethasone at 4mg/d. Main Outcome Measures: Primary outcome measures were gene expression profiling of adipose tissue and skeletal muscle. Urinary cortisol and metabolic biochemistry were also assessed. Overall design: Dexamethasone was provided in 0.5 mg tablets as 4mg per day during 4 days to male healthy volunteers. Skeletal muscle and adipose tissue biopsies were obtained at the beginning and at the end of the protocol. The transcriptome analysis compared before vs. after the 4 days dexamethasone treatment using a dye swap design and whole genome 4x44k oligonucleotide arrays (Agilent Technologies).

Context: 糖皮质激素（Glucocorticoids）为临床常用处方药物，可引发葡萄糖耐受不良、组织重塑等显著不良反应。Objective: 本研究旨在探索短期糖皮质激素治疗期间，脂肪组织与骨骼肌之间交叉对话的分子机制。Design and Intervention: 纳入健康男性受试者，予以地塞米松（dexamethasone）4mg/天，连续给药4天。Main Outcome Measures: 主要结局指标为脂肪组织与骨骼肌的基因表达谱分析；同时检测尿液皮质醇水平及代谢生化指标。Overall design: 本研究为健康男性志愿者制定给药方案：每日给予0.5mg规格的地塞米松片剂，每日总给药剂量为4mg，持续4天。分别于实验开始前及干预结束时采集骨骼肌与脂肪组织活检样本。转录组分析采用染料交换（dye swap）实验设计，使用全基因组4x44k寡核苷酸微阵列（安捷伦科技，Agilent Technologies）对比4天地塞米松给药前后的基因表达差异。