Impact of in vivo acute and chronic interleukin-1 beta treatment on hematopoietic stem cell gene expression

This study identifies molecular changes in hematopoietic stem cells harvested from wild-type C57BL/6 mice following acute or chronic IL-1 exposure in vivo. Overall design: RNA-sequencing analysis of pooled mouse SLAM cells (HSC enriched fraction, defined here as LSK/Flk2-/CD48-/CD150+). Each datafile represents a biological replicate derived from SLAM cells sorted from pools of 3-5 mice.

本研究明确了经体内急性或慢性白细胞介素-1（IL-1）暴露处理的野生型C57BL/6小鼠体内采集的造血干细胞（hematopoietic stem cell）中的分子变化。实验整体设计：对合并的小鼠SLAM细胞（造血干细胞富集组分，本文中将其定义为LSK/Flk2-/CD48-/CD150+）进行RNA测序（RNA-sequencing）分析。每份数据文件对应1份生物学重复，其样本为从3-5只小鼠的混合群体中分选得到的SLAM细胞。