Molecular Mechanisms Driving Switch Behavior in Xylem Cell Differentiation. Molecular Mechanisms Driving Switch Behavior in Xylem Cell Differentiation

Single cell sequencing (DropSeq) from 7 day old Arabidopsis roots with β-estradiol-VND7, MYB83 and MYB46 induction lines and controls Overall design: 7 day Arabidopsis roots either treated with β-estradiol and collected after 24 hours or transferred to control MS plates for 24 hours. All experiments where treated at 7am and collected at 7am the next day for protoplasting and DropSeq

本数据集采用DropSeq技术，对7日龄拟南芥根系的β-雌二醇诱导型VND7、MYB83及MYB46基因过表达株系与对照株系开展单细胞测序。实验整体设计：将7日龄拟南芥根系分为两组，一组经β-雌二醇处理24小时后收集样本，另一组转接至对照MS培养基平板培养24小时后收集样本。所有实验均于清晨7点进行处理，次日清晨7点收集样本，用于原生质体制备及DropSeq测序。