Bos taurus Transcriptome or Gene expression

Transfected miR-150 mimic, mimic NC, inhibitor, and inhibitor NC, bovine adipocytes were used to induce differentiation, and RNA on day 2 and day 6 were collected. A control was set for each treatment, with three technical replicates per group, and a total of 8 groups (24 samples). After RNA extraction, the HiSeq-PE150 platform (Illumina, Sandiego, CA, USA) was used for RNA sequence analysis. The reads after filtering the original data are matched to the bovine genome using HiSat2 (Bos-taurusUMD3.1, release94, Ensembl database), and feature counts are used to counting gene expression.

本研究以转染miR-150模拟物（mimic）、模拟物阴性对照（mimic NC）、抑制剂（inhibitor）及抑制剂阴性对照（inhibitor NC）的牛脂肪细胞为实验材料，诱导其分化，并分别于分化第2天和第6天收集总RNA。每个处理组均设置对照，每组设3个技术重复，共计8组（24个样本）。总RNA提取完成后，采用HiSeq-PE150测序平台（Illumina，美国加利福尼亚州圣地亚哥）进行RNA测序分析。对原始数据过滤后得到的reads，通过HiSat2工具（参考基因组为Bos taurus UMD3.1，版本94，来自Ensembl数据库）比对至牛参考基因组，随后使用feature counts进行基因表达量计数。