原始数据TMYC-2025-0066.xlsx

The basidiomycete <i>Sporisorium scitamineum</i> causes sugarcane smut disease, leading to a significant impairment in the quality and yield of sugarcane. Polyamines affect the intracellular redox balance, which is important for the pathogenic development of <i>S. scitamineum</i>, including sexual mating, dikaryotic hyphae growth, and host invasion. In the present study, we identified a polyamine transporter, SsTPO1, in <i>S. scitamineum</i>. Deletion or overexpression of <i>SsTPO1</i> led to the disruption of intracellular levels of putrescine, spermidine, and spermine in opposite directions. SsTPO1-based polyamine homeostasis contributes to dikaryotic hyphal growth and host invasion after sexual mating, likely by affecting the cAMP-PKA signaling pathway and collaboratively regulating redox homeostasis. Environmental pH affected SsTPO1-mediated polyamine transport, so <i>S. scitamineum</i> hyphae growth favored neutral to alkaline environments rather than acidic conditions. The SsTPO1-based polyamine transport also participates in stress resistance and autophagy induction. In summary, our work revealed that SsTPO1 facilitates polyamine transport to regulate cell growth, differentiation, and fungal pathogenicity in <i>S. scitamineum.</i>

担子菌门（basidiomycete）的甘蔗鞭黑粉菌（*Sporisorium scitamineum*）可引发甘蔗黑穗病，显著损害甘蔗的品质与产量。多胺（polyamines）会影响细胞内氧化还原平衡，而该平衡对于甘蔗鞭黑粉菌的致病发育至关重要，包括有性交配、双核菌丝生长以及宿主入侵。本研究中，我们在甘蔗鞭黑粉菌中鉴定出一种多胺转运蛋白SsTPO1。敲除或过表达*SsTPO1*会以相反方向改变细胞内腐胺（putrescine）、亚精胺（spermidine）和精胺（spermine）的水平。基于SsTPO1的多胺稳态可通过影响cAMP-PKA信号通路并协同调控氧化还原稳态，助力有性交配后的双核菌丝生长与宿主入侵。环境pH会影响SsTPO1介导的多胺转运，因此甘蔗鞭黑粉菌的菌丝生长更倾向于中性至碱性环境，而非酸性条件。基于SsTPO1的多胺转运还参与了胁迫抗性与自噬诱导过程。综上，本研究揭示了SsTPO1可通过介导多胺转运，调控甘蔗鞭黑粉菌的细胞生长、分化与真菌致病性。