Data for: Comparative proteomics of geographically distinct saw-scaled viper (Echis carinatus) venoms from India

We investigated on venom variation in geographically distinct populations of Echis carinatus from three ecologically distinct regions: Tamil Nadu (ECVTN), Goa (ECVGO), and Rajasthan (ECVRAJ). Venom was fractionated by RP-HPLC, combined with SDS-PAGE, and subjected to tandem mass spectrometry. Toxins were identified, and their relative abundance was estimated. Using NCBI database of Echis genus, we queried the MS/MS spectra, and found 87, 44 and 48 proteins, in ECVTN, ECVGO and ECVRAJ respectively, belonging to 7-9 different toxin families. The differences in the venom profiles were due to change in the relative composition of the toxin families.

本研究针对3个生态迥异区域的锯鳞蝰（Echis carinatus）地理隔离种群的毒液变异展开调查，所涉区域分别为泰米尔纳德邦（ECVTN）、果阿邦（ECVGO）与拉贾斯坦邦（ECVRAJ）。研究采用反相高效液相色谱（RP-HPLC）结合十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）对毒液进行分级分离，并通过串联质谱（tandem mass spectrometry）开展分析。随后完成了毒素的鉴定，并对其相对丰度进行了估算。借助锯鳞蝰属（Echis）的美国国家生物技术信息中心（NCBI）数据库，我们对MS/MS谱图进行检索，结果分别在ECVTN、ECVGO及ECVRAJ样本中鉴定得到87、44和48种蛋白质，这些蛋白质隶属于7至9个不同的毒素家族。毒液谱型的差异源于各毒素家族的相对组成发生了改变。