miRNA expression profiles of PRC SiRNA vs scramble transfected XTC.UC1 cells

PGC-1 related coactivator (PRC) shares structural and functional features with PGC-1{alpha}. It regulates several metabolic pathways and mitochondrial biogenesis. Its specific role in the early programming of cell proliferation suggests a finely regulated crosstalk between the mitochondrial functions and the cell cycle status. To explore the PRC-regulated pathways, we used a cell-line model of mitochondrial-rich tumors, presenting an oxidative metabolism and a specific increase in PRC expression. We looked for the feedback loop exerted by miRNAs on the regulation of PRC-related mitochondiral functions Expression profiles of miRNA at 48h PRC SIRNA treatment compared to scramble in XTC.UC1 cells. In duplicate.

PGC-1相关共激活因子（PGC-1 related coactivator, PRC）与PGC-1α在结构与功能上具有高度相似的特征。该因子可调控多条代谢通路及线粒体生物发生过程。其在细胞增殖早期编程中发挥的特异性作用，提示线粒体功能与细胞周期状态之间存在精细调控的信号串扰。为探究PRC所调控的生物学通路，本研究采用了富线粒体肿瘤细胞系模型，该细胞系呈现氧化代谢表型且PRC表达水平特异性升高。本研究旨在分析微小RNA（microRNA, miRNA）对PRC相关线粒体功能的调控反馈环路：以XTC.UC1细胞为实验对象，对比经PRC小干扰RNA与乱序对照siRNA处理48小时后的miRNA表达谱，实验设置重复样本。